Leica DMi8 Stellaris 5 Confocal Laser Scanning Fluorescence Microscope System with AFC Module

For Sale Leica DMi8 Stellaris Confocal Laser Scanning Microscope System with AFC Module

• Fully Functional
• Serviced & in Stock
• Made in 2022
•  Customized to your requirements
•  Includes new fluorescence filters
•  Technical sales consultation available
•  Continental USA 180 DAYS WARRANTY & 60 DAYS INTERNATIONAL
•  Includes remote microscope training session and software overview

Service Highlights

•  Optics quality is checked for field distortion and uniformity at the specimen plane
•  Fluorescence Intensity is also measured at the specimen plane to confirm light source and filter performance
•  Preventive Maintenance (PM) is Completed

This is what you will receive before customizing this microscope:

Standard Leica DMi8 Inverted Microscope Configuration:

Microscopy Techniques:

Brightfield, Polarization, Widefield Fluorescence and Confocal Fluorescence studies

Microscope Base:

• Leica DMi8 Inverted Microscope
  
• CTRadvanced Control Box 
• Stellaris 5 Scanning head
• Stellaris 5 Supply unit

Microscope Head:

• Ergonomic Binocular head
  
• (2) HC PLAN s 10x/25 M Eyepieces

Fluorescence Light Path:

• Motorized external fluorescence axis
• AOBS Stellaris scanning head (Acousto Optical Beam Splitter)

Fluorescence Illumination:

• Leica LED 3 Light Engine (Lumencor)
  
• Stellaris 5 (White laser and 405nm Diode laser)

Fluorescence filter cube turret:

• 6-position Motorized Filter Cube Turret

Fluorescence filters:

• LED 405 filter cube
  
• GFP filter cube
• Rhodamine LP filter cube 
• Brightfield Analyzer cube
• *Other filters available - please inquire*

Brightfield Light Path:

• Tiltable Transmitted Illumination Arm

Brightfield Illumination:

• TL-LED 12V 15W LED Lamp
  
• Leica Fluorescence Protective Cover

Objective Turret:

• 6-position Motorized Objective turret
• Adaptive Focus Control 

Objectives:

• HC PL APO 10x/0,40 CS2
  
• HC PL APO 20x/0,75 CS2
• HC PL APO 40x/1,10 W motCORR CS2
• HC PL APO 40x/1,30 Oil CS2
• HC PL APO 63x/1,40 Oil CS2
• *Other objectives available - please inquire*

Condenser:

• 7-position Motorized S28 0.55 Condenser
• Rotatable Polarizer

Stage:

• Leica Scanning Stage (Original Mfg. Marzhauser)
• Smartmove Joystick
• Tokai Hit STXG Stagetop Incubator

Camera ports:

• 100% Eyepiece
  
• 100% Right Camera Port
• 100% CS Stellaris 5

Camera:

• Stellaris 5 Hyd detectors

Computer & Software:

• Desktop Computer running Leica LAS X software

LAS Software with license dongle for:

• Acquisition License
• Stellaris Base Licence
• 3D Advanced Basic
• 3D Advanced Full
• Deconvolution Expert
• Deconvolution Image Processing
• Dye Finder License
• Assay Editor

Accessories:

• Necessary power and communication cables
• Computer Monitors (2)
• Mouse and Keyboard
• Coolant tubing

Others:

• Leica Control Panel
• Laird MRC 300 Chiller unit
• Newport Anti-Vibration table

Automated/motorized capabilities of this microscope:

Installed automation/motorized components:

• Motorized external fluorescence axis.
• Motorized 6-position fluorescence filter cube turret.
• Motorized 6-position objective turret.
• Motorized 7-position condenser.
• Motorized scanning stage (Marzhauser).
• Motorized Z-axis (integrated in DMi8 platform).
• Automated adaptive focus control.
• Motorized illumination control (LED and laser intensity via system software).
• Motorized port selection (100% eyepiece, right camera port, CS Stellaris 5 port).
• Motorized scanning via Stellaris 5 confocal head.
• Software-controlled laser excitation (white laser and 405 nm diode laser).
• Software-driven filter switching and configuration.
• Automated environmental control system (via stage-top incubator).
• Integrated system control via CTRadvanced control box and Leica LAS X software.

This microscope's imaging capabilities:

Imaging software highlights:

• Leica LAS X software platform for acquisition, control, and analysis.
• Integrated confocal acquisition control for the Stellaris 5 system (Stellaris Base License).
• Advanced 3D imaging capabilities (3D Advanced Basic + Full).
• Z-stack acquisition and reconstruction.
• Automated stitching for large-area imaging.
• Deconvolution tools (Deconvolution Expert + Image Processing).
• Dye Finder module for fluorophore selection.
• Assay Editor for automated workflows.
• Multi-dimensional imaging support (time series, Z, channels, positions).
• Live image acquisition and real-time visualization.
• Customizable acquisition protocols and workflows.

Stellaris 5 Advanced Features:

• Field-of-View Scanner: mirror-based system enabling precise beam positioning with electronic control of offset (panning) and amplitude (zooming) for flexible imaging.
• White Laser Source: iIntegrated white light laser covering 485–790 nm, with a 405 nm diode laser for UV excitation and expanded application range.
• AOBS Beam Splitting (Acousto-Optical Beam Splitter): advanced, non-mechanical beam management system that replaces traditional dichroic-based configurations, improving stability and reducing maintenance.
• Variable Resolution: adjustable scanning resolution from 16 × 16 to 2048 × 2048, supporting both rapid overview scans and high-detail imaging.
• Low-Noise Imaging: optimized system design for high-quality images with minimal background noise and enhanced signal clarity.

AOBS Beam Splitting Technology:

Optical Principle:

• Uses an acousto-optical crystal instead of dichroic mirrors to manage excitation and emission pathways.

System Advantages:

• Reduced mechanical components, resulting in lower maintenance and improved reliability.
• Minimal sample bleaching for gentle imaging of sensitive specimens.
• Broad emission band support for sharper, higher-quality images.
• Narrow transmission bandgaps (2–6 nm) compared to traditional systems (20–80 nm), enabling superior spectral resolution.

Fluorescence Performance:

• Supports up to 8 laser lines (400–800 nm) for multiparameter fluorescence imaging.
• Maintains consistent transmission efficiency as additional laser lines are added.
• Provides individual excitation intensity control for each laser line.
• Enables fast sequential scanning with microsecond switch times.

Imaging Flexibility:

• Compatible with multiphoton and UV imaging in parallel workflows.
• Eliminates alignment-sensitive components such as filter cubes and dichroics.
• Reduces risk of misalignment from mechanical filter sliders or turrets.

We can customize this microscope to fit your requirements:

Increase Maximum Magnification and Image Quality:

• Microscope can be configured for magnification between 100x and 630x (with 10x Eyepieces)
• We can add standard higher-magnification Leica microscope objectives

Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.

• Additional filter cubes
• Additional filter sets to match your fluorochromes.
• Xenon, Metal Halide, or LED Fluorescence microscope light sources

We will also ensure the fluorescence light source matches your selected filter sets.

Enhance imaging capabilities:

• We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.

Add other Motorized/Automation Capabilities:

In addition to installed motorized capabilities, the microscope can be configured

• Motorized transmitted light shutter
• Motorized fluorescence shutter

We can configure it to work with the sample medium you need:

• Slide, Petri dish, microplate, etc.

Commonly Requested Customer Upgrades

• High-End CCD, sCMOS Cameras
• LED or Metal Halide Fluorescence Light Sources
• Phase Contrast, Polarization, or the DIC configurations
• Higher Magnification Objectives
• Higher performance Fluor or APO objectives
• Motorized XY Stage (stage, controller, joystick)

We are excited to help build and configure the right microscope for you!

Leica DMi8 Inverted Microscope Overview

(Final microscope capabilities will depend on the modifications you request)

• Leica DMi8 inverted research microscope integrated with Stellaris 5 confocal scanning for advanced imaging versatility.
• Widefield and confocal fluorescence combined with brightfield and polarization modes for multi-contrast investigations.
• Motorized optical architecture including fluorescence axis, filter turret, objective turret, condenser, and scanning stage.
• AOBS beam splitting technology enabling high transmission efficiency, low photobleaching, and stable multi-laser operation.
• High-performance optomechanics with adaptive focus control and precision HC PL APO objectives for image fidelity.
• Fully integrated control platform with motorized components, scanning head, and environmental stability for live imaging.

Suited for the following applications as configured:

• High-resolution confocal fluorescence imaging and multi-channel colocalization studies.
• Live-cell imaging with environmental control and long-term time-lapse experiments.
• 3D volumetric imaging, reconstruction, and advanced spatial analysis.
• High-content imaging workflows with automated acquisition and multi-position scanning.
• Multimodal microscopy combining brightfield, polarization, widefield, and confocal techniques.
• Quantitative fluorescence imaging with spectral flexibility and optimized fluorophore selection.

Other potential applications:

• Subcellular and structural biology imaging with high spatial resolution.
• Live-cell dynamics and time-lapse studies under controlled conditions.
• 3D imaging and reconstruction of complex biological structures.
• Multiparameter fluorescence assays and quantitative analysis workflows.
• Tissue imaging for histology and biomarker localization.
• Materials and polymer analysis using fluorescence and polarization contrast.

Video Overview:

Sample Overview:

How we pack

REASONS WHY THESE CUSTOMERS CHOOSE TO ORDER FROM US

•   They want to ensure the microscope is customized to fit their application 
•   They want to ensure that internal optics are properly cleaned 
•   They want to ensure that internal mechanical and motorized mechanisms are lubricated and aligned
•   They want to ensure that image quality is verified at the sample point for field distortion and uniformity
•   They want to ensure that the fluorescence intensity is verified at the sample point 
•   They want to ensure NEW fluorescence filters are installed just the same as buying a new microscope
•   They want to ensure that if paired with a motorized XY stage, it is lubricated and does not skip steps
•   They want an option to upgrade the camera to sCMOS and the light source to Lumencor or X-Cite LED
•   They want training to learn how to operate the microscope 
•   They want a reliable warranty
•   They want peace of mind knowing it will be safely and professionally packed for shipping

• We make sure to match the microscope capabilities to your requirements.
• We have experience servicing and repairing these microscopes.
• This microscope goes through an extensive set of service steps before shipping.
• We offer detailed photos and videos of the microscopes you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies. 
• Many upgrade and repair parts are in stock.
• Remote installation and microscope operation training are available.
• On-site installation may be available in the Boston Area (New England area)
• Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)

• Warranty
• An extended warranty may be available.
• You get after-sales technical support.
• Customer references are available.
• The microscope will be complete and working!
• Smooth process from the initial quote to order fulfillment.

COMMON CUSTOMER QUESTIONS AND ANSWERS BELOW